I'm not entirely sure what you want, and your last line of code does not help either, but anyway:

"Chained" filtering is done by "chaining" the criteria in the boolean index.

In [96]: df
Out[96]:
   A  B  C  D
a  1  4  9  1
b  4  5  0  2
c  5  5  1  0
d  1  3  9  6

In [99]: df[(df.A == 1) & (df.D == 6)]
Out[99]:
   A  B  C  D
d  1  3  9  6

If you want to chain methods, you can add your own mask method and use that one.

In [90]: def mask(df, key, value):
   ....:     return df[df[key] == value]
   ....:

In [92]: pandas.DataFrame.mask = mask

In [93]: df = pandas.DataFrame(np.random.randint(0, 10, (4,4)), index=list('abcd'), columns=list('ABCD'))

In [95]: df.ix['d','A'] = df.ix['a', 'A']

In [96]: df
Out[96]:
   A  B  C  D
a  1  4  9  1
b  4  5  0  2
c  5  5  1  0
d  1  3  9  6

In [97]: df.mask('A', 1)
Out[97]:
   A  B  C  D
a  1  4  9  1
d  1  3  9  6

In [98]: df.mask('A', 1).mask('D', 6)
Out[98]:
   A  B  C  D
d  1  3  9  6

You can use pd.Series.isin.

For "IN" use: something.isin(somewhere)

Or for "NOT IN": ~something.isin(somewhere)

As a worked example:

>>> df
    country
0        US
1        UK
2   Germany
3     China
>>> countries_to_keep
['UK', 'China']
>>> df.country.isin(countries_to_keep)
0    False
1     True
2    False
3     True
Name: country, dtype: bool
>>> df[df.country.isin(countries_to_keep)]
    country
1        UK
3     China
>>> df[~df.country.isin(countries_to_keep)]
    country
0        US
2   Germany

Pandas (and numpy) allow for boolean indexing, which will be much more efficient:

In [11]: df.loc[df['col1'] >= 1, 'col1']
Out[11]: 
1    1
2    2
Name: col1

In [12]: df[df['col1'] >= 1]
Out[12]: 
   col1  col2
1     1    11
2     2    12

In [13]: df[(df['col1'] >= 1) & (df['col1'] <=1 )]
Out[13]: 
   col1  col2
1     1    11

If you want to write helper functions for this, consider something along these lines:

In [14]: def b(x, col, op, n): 
             return op(x[col],n)

In [15]: def f(x, *b):
             return x[(np.logical_and(*b))]

In [16]: b1 = b(df, 'col1', ge, 1)

In [17]: b2 = b(df, 'col1', le, 1)

In [18]: f(df, b1, b2)
Out[18]: 
   col1  col2
1     1    11

Update: pandas 0.13 has a query method for these kind of use cases, assuming column names are valid identifiers the following works (and can be more efficient for large frames as it uses numexpr behind the scenes):

In [21]: df.query('col1 <= 1 & 1 <= col1')
Out[21]:
   col1  col2
1     1    11