You need to add your new directory to the environment variable PYTHONPATH, separated by a colon from previous contents thereof. In any form of Unix, you can do that in a startup script appropriate to whatever shell you're using (.profile or whatever, depending on your favorite shell) with a command which, again, depends on the shell in question; in Windows, you can do it through the system GUI for the purpose.

superuser.com may be a better place to ask further, i.e. for more details if you need specifics about how to enrich an environment variable in your chosen platform and shell, since it's not really a programming question per se.

Config files belong in your personal home directory (/home/$USER, $HOME or simply ~), not in the /home directory. In your case that will be /home/john.

Please also make sure to use the correct casing, it's export in all lowercase.

Since export is not accessing but referencing the variable, you do not use the $ sign: export PYTHONPATH

Are you sure you want to have this in your .bash_profile and not your .bashrc? You can read up on the difference here.

In any case you will have to run source ~./bash_profile (or source ~./bashrc if you go with that) for your changes to take effect.

1. execute the command: echo $PATH

   root1@master:/usr/lib/python2.7$ echo $PATH
   /home/root1/anaconda3/bin:/home/root1/NAI/Execution/HDE/x86_64.linux/bin:/usr/local/sbin:/usr/local/bin:/usr/sbin:/usr/bin:/sbin:/bin:/usr/games:/usr/local/games:/home/root1/java/jdk1.8.0_74/bin:/usr/lib/jvm/java-8-oracle/bin:/usr/lib/jvm/java-8-oracle/db/bin:/usr/lib/jvm/java-8-oracle/jre/bin:/home/root1/NAI/hadoop-2.7.3/bin
   

2. Remove your anaconda3 from your path variable that is

   /home/root1/anaconda3/bin:
   

3. Again set PATH variable with remaining information like below

   export PATH=/home/root1/NAI/Execution/HDE/x86_64.linux/bin:/usr/local/sbin:/usr/local/bin:/usr/sbin:/usr/bin:/sbin:/bin:/usr/games:/usr/local/games:/home/root1/java/jdk1.8.0_74/bin:/usr/lib/jvm/java-8-oracle/bin:/usr/lib/jvm/java-8-oracle/db/bin:/usr/lib/jvm/java-8-oracle/jre/bin:/home/root1/NAI/hadoop-2.7.3/bin
   

4. Execute python command and should redirect to your python interpreter

   root1@master:/usr/lib/python2.7$ python
   Python 2.7.14 (default, Sep 18 2017, 00:00:00) 
   [GCC 4.8.4] on linux2
   Type "help", "copyright", "credits" or "license" for more information.
   >>> 
   

I'd like to summarize my findings about python's path modification. There are two ways to do it.

• .pth file
• PYTHONPATH

Any .pth file which is found on the default path (see bellow) will get its content included into sys.path. Format of said .pth file is simple: one (folder) path per line. Surprisingly, the paths can be absolute or relative to the .pth file.

Default path is where the interpreter resides and <some-prefix>/lib/python<version>/site-packages where <some-prefix> is usually /usr/.

PYTHONPATH is environmental variable of your operating system. On unix systems you list them by env. Global modification of such variables is done through .sh scripts inside /etc/profile.d/ folder as mentioned by @TestUser16418.