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April 27, 2023 - Here, we present nanopore dimethylsulfate mutational profiling (Nano-DMS-MaP)—a method that exploits long-read sequencing to provide isoform-resolved structural information of highly similar RNA molecules.

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Analysis pipeline for RNA structural probing mutational profiling data sequenced on Nanopore devices - smyth-lab/Nano-DMS-MaP

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August 14, 2024 - This Protocol outlines the development and applications of Nano-DMS-MaP, providing a roadmap for molecular biologists to conduct in situ isoform-specific RNA structure determination experiments.

October 11, 2024 - DMS-MaP is a sequencing-based method for assessing RNA folding by detecting methyl adducts on unpaired A and C residues created by treatment with dimethylsulfate (DMS). DMS also creates methyl adducts on the N7 position of G, which could be sensitive to tertiary interactions with that atom, ...

February 12, 2024 - A protocol for Nano-DMS-MaP, a method for in situ isoform-specific RNA structure determination. The technique employs nanopore sequencing to resolve the structures of long and highly similar RNA molecules, revealing previously hidden structural ...

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April 9, 2026 - Several methods have been developed to profile in vivo RNA secondary structure in prokaryotes and eukaryotes. These methods, such as dimethyl sulfate (DMS) mutational profiling with high-throughput sequencing (DMS-MaPseq), couple small chemical-mediated RNA modifications with next-generation sequencing.

November 28, 2024 - We report a new chemical probing strategy termed multi-site DMS-MaP (msDMS-MaP) that exploits base tautomerization during mutational profiling reverse-transcription to measure typically invisible DMS-induced modifications at the N7 position of G. ...

April 22, 2025 - By systematically assessing various RT enzymes and buffers, we optimize conditions that minimize drop-off and enhance error signatures linked to RNA structure. Our results demonstrate that DMS-FIRST-seq generates high-resolution, accurate RNA structure maps in both in vivo and in vitro contexts, thus providing a simple and cost-effective method for transcriptome-wide isoform-specific RNA structural analyses.

2 weeks ago - The Digital Mapping System (DMS) is a removable airborne digital camera system that acquires high resolution natural color and panchromatic imagery from low and medium altitude research aircraft.

The Digital Mapping System (DMS) is an airborne digital camera system that acquires high resolution natural color and panchromatic imagery from low and medium altitude research aircraft. The DMS includes an Applanix Position and Orientation system to allow precision image geo-rectification.

February 19, 2026 - Current RNA structural probing methods, such as DMS-mutational profiling sequencing (DMS-MaP), use NGS technologies whose short reads cannot be reliably mapped to specific transcripts for shared regions of transcript isoforms.

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November 29, 2018 - Numerous methods that integrate ... mutational profiling through NGS (DMS-MaPseq) is a newly developed method for revealing genome-wide or target-specific RNA structure....