I'm not sure I understand your question. I assume you are doing something along the lines of:

import base64

with open("yourfile.ext", "rb") as image_file:
    encoded_string = base64.b64encode(image_file.read())

You have to open the file first of course, and read its contents - you cannot simply pass the path to the encode function.

Edit: Ok, here is an update after you have edited your original question.

First of all, remember to use raw strings (prefix the string with 'r') when using path delimiters on Windows, to prevent accidentally hitting an escape character. Second, PIL's Image.open either accepts a filename, or a file-like (that is, the object has to provide read, seek and tell methods).

That being said, you can use cStringIO to create such an object from a memory buffer:

import cStringIO
import PIL.Image

# assume data contains your decoded image
file_like = cStringIO.StringIO(data)

img = PIL.Image.open(file_like)
img.show()
Answer from Jim Brissom on Stack Overflow
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GitHub
gist.github.com › juliensalinas › 15789d241f28b1ce45f0c22e11ba894a
Encoding a file in base 64 in Python · GitHub
Encoding a file in base 64 in Python · Raw · base64_encode.py · This file contains hidden or bidirectional Unicode text that may be interpreted or compiled differently than what appears below.
Discussions

Base 64 Encode Python Files!
Nice, but base64 is not encryption, it won't stop people from getting the data. A better use would be when sending your programs or non ascii files though a medium, which can mess up formatting or doesn't support certain data types (pastebin, minecraft's book and quill). More on reddit.com
🌐 r/learnpython
6
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October 20, 2018
Python 3 and base64 encoding of a binary file - Stack Overflow
I'm new to Python and I do have an issue that is bothering me. I use the following code to get a base64 string representation of my zip file. with open( "C:\\Users\\Mario\\Downloads\\exportTest1.... More on stackoverflow.com
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Stupid Linux tricks - use base64 to perfectly preserve formatting when copy/pasting between terminals, ssh sessions, serial connections, etc.
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85
379
April 29, 2023
Converting Base64 file back to Excel
This seems to be what you want? https://stackoverflow.com/a/38935990/409025 More on reddit.com
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2
6
February 25, 2022
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Python
docs.python.org › 3 › library › base64.html
base64 — Base16, Base32, Base64, Base85 Data Encodings
The legacy interface does not support decoding from strings, but it does provide functions for encoding and decoding to and from file objects. It only supports the Base64 standard alphabet, and it adds newlines every 76 characters as per RFC 2045.
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Stack Abuse
stackabuse.com › encoding-and-decoding-base64-strings-in-python
Encoding and Decoding Base64 Strings in Python
September 4, 2023 - Python 3 provides a base64 module that allows us to easily encode and decode information. We first convert the string into a bytes-like object. Once converted, we can use the base64 module to encode it. In a new file encoding_text.py, enter the following:
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iO Flood
ioflood.com › blog › python-base64-encode
Python Base64 Encode | b64encode() Function Guide
February 1, 2024 - The b64encode() function uses the standard base64 alphabet, while urlsafe_b64encode() uses a URL and filename safe base64 alphabet. ... Both methods have their own advantages and use cases.
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Envato Tuts+
code.tutsplus.com › home › coding fundamentals › oop
Base64 Encoding and Decoding Using Python | Envato Tuts+
April 11, 2022 - The first thing we have to do in order to use Base64 in Python is to import the base64 module: ... In order to encode the image, we simply use the function base64.b64encode(s).
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Reddit
reddit.com › r/learnpython › base 64 encode python files!
r/learnpython on Reddit: Base 64 Encode Python Files!
October 20, 2018 -
import base64, os
path = ""
path2 = ""
flag = False
while not os.path.isfile(path):
    if flag:
        print("File path does not exist!\n")
    path = input("Enter path to python file to base64 encode: ")
    flag = True
    
path2 = input("Enter path to save base64 encoded python file: ")
if os.path.isfile(path):
    contentb64 = ""
    with open(path, "r") as file:
        content = file.read()
        contentb64 = base64.b64encode(content.encode()).decode()

    contentb64 = 'import base64; exec(base64.b64decode(b"%s").decode())' % contentb64
    with open(path2, "w") as file:
        file.write(contentb64)

    print("dOnE!")
    input(); exit()

Base 64 encodes python code, can be useful if you don't want people to see your code, just modify this one to do it multiple times as you wish.

Find elsewhere
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GeeksforGeeks
geeksforgeeks.org › python › encoding-and-decoding-base64-strings-in-python
Encoding and Decoding Base64 Strings in Python - GeeksforGeeks
August 7, 2024 - The below image provides us with a Base64 encoding table. ... In Python the base64 module is used to encode and decode data. First, the strings are converted into byte-like objects and then encoded using the base64 module.
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DEV Community
dev.to › tooleroid › base64-encoding-in-python-simplified-guide-examples-nan
Base64 Encoding in Python: Simplified Guide & Examples - DEV Community
December 16, 2024 - Encode files like images and videos for APIs. Safeguard binary data in text-based formats like JSON or XML. Decode received Base64 strings back into usable binary data. Python’s base64 module includes b64encode for encoding text or binary data.
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AskPython
askpython.com › home › encoding an image file with base64 in python
Encoding an Image File With BASE64 in Python - AskPython
April 29, 2023 - To encode an image, first, import the base64 module. Then, open the image file in binary mode and read its content. Use the base64.b64encode() function to convert the image content into a Base64 string.
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Python Module of the Week
pymotw.com › 2 › base64
base64 – Encode binary data into ASCII characters - Python Module of the Week
import base64 original_string = ...ded_string) print 'Decoded :', decoded_string · The encoding process looks at each sequence of 24 bits in the input (3 bytes) and encodes those same 24 bits spread over 4 bytes in the output....
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YouTube
youtube.com › neuralnine
Base64 Encoding Binary Files in Python - YouTube
In this video we learn how to encode binary data or files using Base64 in Python.◾◾◾◾◾◾◾◾◾◾◾◾◾◾◾◾◾📚 Programming Books & Merch 📚🐍 The Python Bible Book: ht...
Published   June 1, 2023
Views   18K
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W3Schools
w3schools.com › python › ref_module_base64.asp
Python base64 Module
Python Examples Python Compiler ... · ❮ Standard Library Modules · Encode and decode a string using Base64: import base64 s = "Linus".encode() b = base64.b64encode(s) print(b) print(base64.b64decode(b).decode()) Try it ...
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MojoAuth
mojoauth.com › binary-encoding-decoding › base64-with-python
Base64 with Python | Binary Encoding Techniques Across Programming Languages
Base64 encoding is incredibly useful for transmitting binary data, like images or other files, over mediums that primarily handle text. In Python, you can easily encode file content into Base64 and then decode it back.
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Cybrosys Technologies
cybrosys.com › odoo blogs
Base64 Encoding & Decoding Using Python
July 18, 2023 - The Python base64 module provides an easy way to encrypt and decrypt binary data using the Base64 encoding technique. It is also extremely simple to use.
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Base64.Guru
base64.guru › home › developers › python
Python Base64 Encode | Python | Developers | Base64
data (required string) - Text or binary content that you want to encode · altchars (optional string; default None) - Two characters that should replace “+” and “/” · str - On success, it returns the Base64 value, typically longer by about 33% than data ... from base64 import b64encode data = 'guru' print(b64encode(data)) #-> 'Z3VydQ==' SyntaxError: bytes can only contain ASCII literal characters. Example #2 (fix the “TypeError: a bytes-like object is required, not 'str'” error on Python 3):
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Base64Encode.org
base64encode.org › enc › python
Base64 Encoding of "python" - Online
For the files section, this is partially irrelevant since files already contain the corresponding separators, but you can define which one to use for the "encode each line separately" and "split lines into chunks" functions. Encode each line separately: Even newline characters are converted to their Base64-encoded forms.
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Elc
elc.github.io › python-security › chapters › 92_Base64.html
Base64 Encoding — Python Security
zip_decoded = base64.urlsafe_b... else: print("The extracted file is NOT the same") ... Base64 is an encoding mechanism that allows to convert to string long sequences of bytes, it has two supported implementations in Python, standart and urlsafe....