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The standard error (SE) of a statistic (usually an estimator of a parameter, like the average or mean) is the standard deviation of its sampling distribution. The standard error is often used … Wikipedia
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Standard error - Wikipedia
October 10, 2025 - This is because as the sample size ... deviation is such that, for a given sample size, the standard error of the mean equals the standard deviation divided by the square root of the sample size....
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What Is Standard Error? | How to Calculate (Guide with Examples)
June 22, 2023 - The standard error of the mean, or simply standard error, indicates how different the population mean is likely to be from a sample mean. It tells you how
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confidence interval - What does standard error of the mean ACTUALLY show? - Cross Validated
I then repeat this process 100 times. By the end of the experiment, I will have 100 means, one for each time I sampled the population. This is the sampling distribution of the mean. As I understand it, the standard deviation of this sampling distribution is the standard error of the mean. More on stats.stackexchange.com
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June 10, 2020
Statistics question: Why is the standard error, which is calculated from 1 sample, a good approximation for the spread of many hypothetical means? - Cross Validated
So, the SE calculated from 1 sample "shouldn't" be far off from the spread of many means? I'm obviously paraphrasing here, but just trying to get a general idea. $\endgroup$ ... As I understand it, the standard error is the spread of many sample means in an attempt to gauge how precise (not ... More on stats.stackexchange.com
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October 24, 2021
Sampling Error vs Standard Error of the Sample Mean - CFA Level I - AnalystForum
Essentially, its the difference that results in inherent differences between the sample and population. For a standard error of the sample mean, is this referring to the standard deviation of the sample mean (ie. with x% confidence and the standard error, you can reject the null hyp... More on analystforum.com
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March 4, 2010
How does the standard error work? - Cross Validated
I have been looking into the inner-workings of the standard error recently, and I found myself unable to understand how it works. My understanding of the standard error is that it is the standard deviation of the distribution of sample means. More on stats.stackexchange.com
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August 2, 2012
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What is standard error?
The standard error of the mean, or simply standard error, indicates how different the population mean is likely to be from a sample mean. It tells you how much the sample mean would vary if you were to repeat a study using new samples from within a single population.
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What’s the difference between standard error and standard deviation?
Standard error and standard deviation are both measures of variability. The standard deviation reflects variability within a sample, while the standard error estimates the variability across samples of a population.
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What Is Standard Error? | How to Calculate (Guide with Examples)
What’s the difference between a point estimate and an interval estimate?
Using descriptive and inferential statistics, you can make two types of estimates about the population: point estimates and interval estimates. Β· A point estimate is a single value estimate of a parameter. For instance, a sample mean is a point estimate of a population mean. Β· An interval estimate gives you a range of values where the parameter is expected to lie. A confidence interval is the most common type of interval estimate. Β· Both types of estimates are important for gathering a clear idea of where a parameter is likely to lie.
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r/statistics on Reddit: [Q] Explain to me how Standard Error is able to do what it does?
October 15, 2021 -

My understanding is that standard error is essentially a measure of how different the means you obtain when you sample from a population will be. According to statistical theory, if you have a population, and you take a sample of this population, you can calculate standard deviation by comparing each value to the mean of your sample. But then, when you take that number and simply divide it by the square root of your sample size, then voila, you magically know how spread out the mean of every single sample you could ever take of that population is.

To me, that seems like a HUGE stretch that you can make such a huge assumption. It is already a bit of a stretch to think that your sample is a decent representation of an actual population mean, and sure, I get that these formulas are actually just estimates rather than concrete math. But I never would have guessed that the deviation of a sample, divided by a modification of the sample size, could tell you how much any mean sample could ever vary, ever.

Am I way off in assuming this? Am I missing something that should make me think more clearly about this all?

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Standard Error of the Mean (SEM) - Statistics By Jim
June 24, 2025 - That’s not good when you’re using sample means to estimate population means! You want narrow sampling distributions where sample means fall near the population mean. The variability of the sampling distribution is the standard error of the mean!
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Maths and Stats - Variance, Standard Deviation and Standard Error - LibGuides@Southampton at University of Southampton Library
November 10, 2025 - The most common standard error is the standard error of the mean, and used to measure sampling error as it measures how accurately the mean of a sample distribution represents the mean of the population.
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Standard Error of the Mean | CFA Level 1 - AnalystPrep
The standard error (SE) of the sample mean refers to the standard deviation of the distribution of the sample means. It gives analysts an estimate of the variability they would expect if they were to draw multiple samples from the same population.
Published Β  February 11, 2025
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What is the Standard Error of a Sample ? - Statistics How To
May 12, 2024 - In statistics, you’ll come across terms like β€œthe standard error of the mean” or β€œthe standard error of the median.” The SE tells you how far your sample statistic (like the sample mean) deviates from the actual population mean.
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How to calculate standard error of mean?
December 9, 2021 - SEM represents an estimate of standard deviation, which has been calculated from the sample. The formula for standard error of the mean is equal to the ratio of the standard deviation to the root of sample size.
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Standard Error of Sample Means
The means of samples of size n, ... the source population and whose standard deviation ("standard error") is equal to the standard deviation of the source population divided by the square root of n....
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Standard Error (SE) Definition: Standard Deviation in Statistics Explained
May 16, 2025 - Standard error is the approximate standard deviation of a statistical sample population. The standard error describes the variation between the calculated mean of the population and one which is considered known, or accepted as accurate.
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Standard Error of the Mean vs. Standard Deviation
March 24, 2025 - Standard deviation describes how ... error of the mean (SEM) indicates how accurately a data set represents the true population by comparing the dataset's average to the population's average....
Top answer
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I agree that the nomenclature and the formulas for the standard mean can be confusing. (Not complicated, actually, just confusing.) Our highly-voted threads in the "standard-error" tag may be enlightening.

I'll take your question step by step.

Imagine I have a population of 500 strings. I randomly sample 20 strings from this population, measure their lengths, and calculate the sample mean. I then repeat this process 100 times. By the end of the experiment, I will have 100 means, one for each time I sampled the population. This is the sampling distribution of the mean.

Correct!

As I understand it, the standard deviation of this sampling distribution is the standard error of the mean. We want the standard error of the mean to be small as it means we are better zeroed in on the true population mean.

Also correct! This is actually the definition of the standard error of the mean (or actually of any estimated parameter!): any parameter estimate will have a distribution, and the standard deviation of this distribution is defined to be the standard error of the parameter.

However, the standard error of the mean is also expressed as the ratio of the standard deviation of the population to the square root of the sample size (here, 20). Furthermore, it can be estimated as the ratio of the standard deviation of a single sampling of 20 strings to the square root of that sample size (again, 20).

Almost. The first statement is not an alternative definition of the SE. It is a mathematical equality that holds under certain assumptions (which are in practice usually fulfilled) that the SE of the mean is equal to $\frac{\sigma}{\sqrt{n}}$. And the second statement is correct: you can estimate the SEM by using an estimate $\hat{\sigma}$ of $\sigma$.

So my question is, how does the second definition using only the standard deviations of the population or sample along with the sample size connect to the original definition in which standard error of the mean is defined as the standard deviation of our sampling distribution? I can't wrap my head around the connection.

As above: that the two are equal is not a question of competing definitions. It's a question of having one definition (as above) and a mathematical theorem than the SEM so defined is equal to $\frac{\sigma}{\sqrt{n}}$.

For instance, as we conduct more and more samplings, the standard deviation of the resulting sampling distribution will continue to decrease more and more, right? So how is this fact taken into account in the equation that only uses standard deviation of a single sample divided by that sample size? Surely the standard deviation of the sampling distribution (which is the standard error!) consisting of 20000000 means will be smaller than the value we get if we simply calculate it by taking the ratio of a single sample standard deviation to the sqrt of the sample size, right?

No. The sampling distribution of the mean depends on the distribution of the original data and on how many observations each separate mean is calculated from (i.e., $n$). It does not depend on how often you sample n points and calculate a mean. This is just drawing more and more samples from the sampling distribution of the mean. The SD of these samples will not decrease just because you draw more and more often.

Simulations in R are a great tool to understand stuff like this. For instance, you could draw 100, 1000, 10000, ... means, each based on $n$ observations from the original data, and you could observe that the SD of the means does not budge a lot. For instance, here are the standard deviations of 10, 50, 100, 500, 1000, 5000, 10000 means, each one based on $n=20$ observations of the original population. It's a flat line, up to variability (meta: we could also investigate the standard error of the estimate of the standard error of the means, but I don't think we want to go there right now...):

R code:

set.seed(1) # for reproducibility
string_lengths <- runif(500)
nn <- 20
n_means <- c(10,50,100,500,1000,5000,10000)
sds <- sapply(n_means,function(kk)sd(replicate(kk,mean(sample(string_lengths,nn,replace=TRUE)))))
plot(n_means,sds,type="o")

Using the second definition, we are calculating standard error by looking at a single sample consisting of 20 measurements. But this isn't even a sampling distribution of the mean, but rather a point estimate of the mean. So how is it possible for it to even have a standard error when it's just ONE estimate?

Per above: the standard error is not a property of an observation, but of a distribution. And we can happily estimate it from a single observation of the distribution of the means... because this single observation is in turn based on $n$ observations from the underlying distribution of the original data!

Suppose I have a single string. I then measure that string 20 times. That's it.

Question three: In this experiment, there isn't really a 'population' from which I'm sampling. I'm just measuring the same string over and over. So how am I supposed to calculate a standard error from this? If each sampling has sample is size one, then its impossible to calculate any means nor any sampling distribution of those means. Alternatively, if we assume the 20 measurements belonged to a SINGLE sampling, then I'm still not able to construct a sampling distribution of the means, since I only got ONE mean. Sure, I could calculate the standard error of the 20 measurements, but that's not standard error, it's just the standard deviation!!

Well, if you just wrote down a single observation 20 times, then you can't estimate the population standard deviation $\sigma$, because you have only one observation. (Technically, you have 20 observations, but they are not independent, which is one of the technical conditions I mentioned above. If your conditions are not met, of course all bets are off.) So in this situation, there is really nothing you can't do, and theory won't help you.

(Incidentally, there is a population you are sampling from. It may be the 500 strings we started out with, or it could be just a single one, but we always have a population. We are just not sampling from it independently.)

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As I understand it, the standard error is the spread of many sample means in an attempt to gauge how precise (not accurate) our estimate of the population mean is, but what if there's just the one sample?

Very short

A sample is not just one sample but contains many individual observations. Each of the observations can be considered as a sample (is there a difference between '$n$ samples of size 1' and '1 sample of size $n$'?). So you actually have multiple samples that can help to estimate the standard error in sample means.

In order to estimate the variance of the mean of samples, would you rather have a sample of size one million or multiple (say a hundred) samples of ten?

A bit longer

A sample will almost never be picked such that it perfectly matches the population. Sometimes a sample might pick relatively low values, sometimes a sample might pick relatively high values.

The variation in the sample mean, due to these random variations in picking the sample, is related to the variation in the population that is sampled. If the population has a wide spread in high and low values, than the deviations in a random samples with relatively high/low values will be corresponding to this wide spread and they will be large.

The error/variation in the means of samples relates to the variance of the population. So we can estimate the former with the help of an estimate of the latter. We can estimate the variance of sample.means by the variance of the population. And for this estimate of the variance of the population, one single sample is sufficient.

In formula form

The variance of the sample means $\sigma_n$ where the samples are of size $n$ is related to the variance of the population $\sigma$ $$\sigma_n = \frac{\sigma}{\sqrt{n}}$$

So an estimate of $\sigma$, for which a single sample is sufficient, can also be used to estimate $\sigma_n$.

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I propose to put some visuals/intuition to your question... using an empirical approach (bootstrapping) to make it more concrete, especially in reference to the following:

Usually experiments can't or just aren't repeated and only have 1 sample from a population

As you highlighted it, we are talking about the standard error of a statistic (the mean in our case). So, Let's assume that you have a random sample of 20 people's height from a given country:

##  [1] 192.3214 144.4797 151.3796 155.2519 147.5844 147.9056 171.1867 159.3074
##  [9] 163.0097 190.9857 165.8155 198.2192 192.2418 165.3628 186.9498 167.3355
## [17] 148.6400 156.6933 160.8472 174.4827

From this sample, you get a mean of 167 and a standard deviation of 17.

You have only one random sample, but you can imagine that if you could take another one, you might get similar values, sometimes duplicates or sometimes more extreme values... but something that will look like to your initial random sample.

So, from these initial sample values and without inventing new ones (only resampling with replacement), you can imagine many other samples. For example, we can imagine three as follows:

##  [1] 165.8155 159.3074 148.6400 165.3628 155.2519 151.3796 192.2418 163.0097
##  [9] 159.3074 192.2418 186.9498 163.0097 144.4797 198.2192 159.3074 190.9857
## [17] 165.3628 159.3074 167.3355 156.6933
##  [1] 147.5844 147.9056 151.3796 163.0097 167.3355 159.3074 167.3355 156.6933
##  [9] 156.6933 159.3074 147.9056 190.9857 192.2418 171.1867 198.2192 147.9056
## [17] 155.2519 167.3355 148.6400 165.8155
##  [1] 192.2418 198.2192 156.6933 192.3214 148.6400 192.3214 198.2192 165.8155
##  [9] 167.3355 144.4797 163.0097 148.6400 159.3074 163.0097 163.0097 174.4827
## [17] 165.3628 165.8155 174.4827 159.3074

Their respective mean will be different from the initial one... but what is interesting is that if we repeat this resampling exercise 10,000 times, for instance, and we calculate the mean for each of these generated samples, we will get something like that (leaving the R code here, just to illustrate it), a distribution of means centered around the initial sample mean:

set.seed(007)

spl <- 167+17*scale(rnorm(20))[,1]  #Forcing to have same mean and sd for all samples

library(boot)
myFunc <- function(data, i){
  return(mean(data[i]))
}

bootMean <- boot(spl , statistic=myFunc, R=10000)
hist(bootMean$t, xlim=c(150,185), main="Sample size n=20")
abline(v=mean(spl), col="blue")

So, the histogram above represents the distribution of means of 10,000 samples… that we constructed from our initial sample. Empirically, we can determine the standard deviation of this (sampling) distribution (which is our standard error of the mean):

sd(bootMean$t)
## [1] 3.74095

Interestingly enough, if we calculate the formula for the standard error $\frac{s}{\sqrt n}$, we get something very similar:

sd(spl)/sqrt(20)
## [1] 3.801316

The standard error of the mean tells us about the spread our data around the mean.

To finish this intuitive overview, let's see what happen if we increase our initial sample size (to understand the impact of this $\sqrt{n}$).

So, if we increase the sample size, the standard error gets unsurprisingly smaller... we reduce the error in estimating the population mean. Again, we can empirically see that the formula still holds:

sd(bootMean$t)
## [1] 0.7740625
sd(spl)/sqrt(500)
## [1] 0.7602631
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Sampling Error vs Standard Error of the Sample Mean - CFA Level I - AnalystForum
March 4, 2010 - For a standard error of the sample mean, is this referring to the standard deviation of the sample mean (ie. with x% confidence and the standard error, you can reject the null hyp...
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How To Find The Standard Error: Formula & Calculation
July 31, 2023 - Standard error is calculated by dividing the standard deviation of the sample by the square root of the sample size. Calculate the mean of the total population.
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The series of means, like the series of observations in each sample, has a standard deviation. The standard error of the mean of one sample is an estimate of the standard deviation that would be obtained from the means of a large number of samples drawn from that population.
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Statistical notes for clinical researchers: Understanding standard deviations and standard errors - PMC
The distribution of all possible ... (true) population value if the distribution is approximately normal. The standard error is the standard deviation of a sampling distribution....
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What Is Standard Error? Statistics Calculation and Overview | Outlier
April 13, 2023 - A large standard error tells you that the sampling distribution is widely spread around its mean, while a smaller standard error tells you that the sampling distribution is more tightly clustered around the true population mean. The smaller the standard error is, the more likely it is that any given sample mean you calculate is closer to the value of the true population mean.
Top answer
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Yes, the standard error of the mean (SEM) is the standard deviation (SD) of the means. (Standard error is another way to say SD of a sampling distribution. In this case, the sampling distribution is means for samples of a fixed size, say N.) There is a mathematical relationship between the SEM and the population SD: SEM = population SD / the square root of N. This mathematical relationship is very helpful, since we almost never have a direct estimate of the SEM but we do have an estimate of the population SD (namely the SD of our sample). As to your second question, if you were to collect multiple samples of size N and calculate the mean for each sample you could estimate the SEM simply by calculating the SD of the means. So the formula for SEM does indeed mirror the formula for the SD of a single sample.

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Suppose $X_1, X_2, \ldots, X_n$ are independent and identically distributed. This is the situation I am pretty sure you are referring to. Let their common mean be $\mu$ and their common variance be $\sigma^2$.

Now the sample mean is $X_b=\sum_i X_i/n$. Linearity of expectation shows that the mean of $X_b$ is also $\mu$. The independence assumption implies the variance of $X_b$ is the sum of the variances of its terms. Each such term $X_i/n$ has variance $\sigma^2/n^2$ (because the variance of a constant times a random variable is the constant squared times the variance of the random variable). We have $n$ identically distributed such variables to sum, so each term has that same variance. As a result, we get $n \sigma^2/n^2 = \sigma^2/n$ for the variance of the sample mean.

Usually we do not know $\sigma^2$ and so we must estimate it from the data. Depending on the setting, there are various ways to do this. The two most common, general-purpose estimates of $\sigma^2$ are the sample variance $s^2 = \frac{1}{n}\sum_i(X_i-X_b)^2$ and a small multiple of it, $s_u^2 = \frac{n}{n-1}s^2$ (which is an unbiased estimator of $\sigma^2$). Using either one of these in place of $\sigma^2$ in the preceding paragraph and taking the square root gives the standard error in the form of $s/\sqrt{n}$ or $s_u/\sqrt{n}$.